Apparatus for making vaccine



flpeetiou @multim-eh all whom t may; concern.' e it lmowu thet L liilrmeuu i. 'l'.umeoN, time ofthe United tetee, resident of uueupolis, cuuuty o' Hennepin, Stute of uuesote. here invented certain new und l" l improvements iu Apparatus fort/lukor.' which the 'oiowiug is u,

generally iu the epfie-if described und particular-- ui the elimine.

eomgijinyiug drawings 'forming pecihcati M, e represents e longitudinal secoi the upimretus embodying my n; i i,

J e ligur iiouul View iii reutieu.

the druiyihgv 2 represeeas sec-tion ei the well ol? u tuut; in which u gee, suitzibie ir the iguivpoee7 under e. high pressure is outniued The which l. prefer to emvloy for this purpose is CU., A Cep 3 is "upped into this tank end iuteriorly. .,hrmuled to .reserve the exteriorly threaded; mul o'i :i metal tube ab preferably' steel, heviug :i lining.; preferably of tin. A port 6 leads tromV the tuuk into the lower end of this tube. 7 vis a compression spring seated in the bottom of the tube and 8 is im ordinary glgiss'teet tube that. is seated on the Spring und havin'r its Wells spaced from the lining of the tube and extending to e point meer the top' of thetube. This test zfube partially 4lilled with tiuid containing iiueierie. 5) is heed hui/ing u recess lO- in its lower end, iuteriorly threaded to receive i? exteriorly ti'ireaded upper end of the time l und bdrm u 'eiosed joiut therewith.

boou et the recess has u socket ll -l' in with u l2 surrounding the eed of the Hochet aud a. concave ifi :it the upper ,aud ei the Boeket, from leeds to u chamber l5 that iu tue upper end oi? the head 9,

.t ,i6 being;r provided 'around the o'peu Ci' Wi u, cap- 18 und projects ie ei'iarube ,l5 und has e.: seating ou tbe eurliuee und eieutueily cieeiug hmuglr A tube4 pre'b gse yu e wie ubit@ will. Serial Ne. llji.

erubly glues; isi-litted into the socket il .und 'Thus its upperehd Seated-'in the bottomeif "the soeket and its lowereud resting upon .the bottom of the test tube 8 but Without in rea geod connection between the heed aud the cup when the vulve l? eentects with the lsurface around and eloses'the port 14. A groyity outlet 2Q is provided iu the bettomot' the chzuuber l5 to which e smell spout 21 is connected for collecting' the uid from the chamber l5 into a steriletest tube.

in using' the upmrutug the bacterie to be studied ure placed in u sterile test tube res ing ou the coiled spring,f in the bottom of' the tiri lined steel tube, the receiving chember iu the heed 9 beingl closed. yThe ugipurutus is then attached to the gus tank und allowed te remain `for a suitable period. During this periud the gas will ,ilow into the test tube 'und being soluble iu the wuter, will mix therewith. Upon the slight opening' of the vulve l? the pressure of the gas will force the bacterial Suspension up the glues tube i3 and through the port into the receiving chamber aud as the fluid passes the vulve, the pressure will suddenly released withiu the-chamber und the bacteria will be Vdestroyed either directly, by the pressure or its sudden release, or possibly bv u combina time of both. The valve is under such coutrel that the fluid may be removed drop by drop as desired und es it fiows into the bottom of the chamber l5 it will settle by gruyity through. the port into the spout 2l aud thence tothe test tube.

lt is ebvious that the details of the censtructiou herein shown und described may be modified in lulu/Ly particulars witb'outdeparting; from my invention es set forth in. the 'following claims..

u. my eO-peudiug application Serial No. 225,055 tiled lifi'urch 27, 1,91%, process oi'moking vaccine, l here described zuid claimed u process; which muy be cui-rieti out by meuus of un eppurutus or device like that dis-- closed 'and claimed herein.

'l claim as my invention:

. l. Ain apparatus of the clues describe( eomprising'u receptacle 4For fluid containing 2. lAn apparatus of the class described, comprising a casing having means for connection with gas under high pressure, a test tube fitting Within seid casing to contain bacteria suspended in fluid in which gas is soluble, said easing having a gas releasing chamber and inlet and outlet ports therefor,

and means for normally losing said inlet port, said inlet port having means submerged .in said fluid for conducting the mingled gas and fiuid to said port for release in said ebaniber when said closing means is opened.

An apparatus of the class described comprising a casing having means for conneetion with gas under high pressure, a test tube for fluid having bacteria suspended therein fitting within said casing and in which fluid said gas is soluble, said. easing having:r a gas releasing chamber With inlet and outlet perfs, a tube for said inlet port submerged in said fluid, means for normally closing said inlet port, the mingled fluid and gas flowing thereto and being released in said chamber when said closing' means is opened.

4. An apparatus of the class desrribed comprising a metallic tube having means f or connection with a suitable under high pressure, a test tube containing fluid having bacteria suspended therein fitting.;- within said metallic tube, a spring; norn'ialli7 holding said test tube iu its raised position. a

passage being provided between tlie walls'ot said test tube and said met-allie tube for admitting the gas pressure to said fluid, a head mounted on said metallic lube and havinga, gas releasing elu'unber provided with inlet and outlet ports and means for normally closing said inlet port.y and a tube emnmunieating at its upper end with said inl= L port and having its louer und seated in sziid tes tube withinthe fiuid therein, the min eu gas and fluid flowing through said inletI port and being released in said chamber when said closing means is opened.

5. An. apparatus of the Class described comprising a receptacle for fiuid eiinlainin;r bacteria havingn means for conuevtiou will gas under pressure and soluble inl aid fluid, and a gas expansion. chambre.' hanno.r :nenne` lfor admitting mingled gas and bar-ferial fluid thereto, for the purpose specified.

In Witness whereof", I have hereuno set my hand this 1Q day of. hai'areh 1018. 

